yppG [2019-07-03 12:14:48]

spore coat protein

locus: BSU22250

pI: 9.51

mw: 14.40 kDa

protein length: 125 aa

gene length: 378 bp

function: protection of the spore

product: spore coat protein

essential: no

synonyms

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.1|Spore coat proteins] → [category|SW 4.2.1.1.5|Class V]

Gene

Coordinates: 2,338,017 → 2,338,394

The protein

Protein family

• NudF subfamily (according to Swiss-Prot)

[SW|Localization]

• spore coat (basement), localization depends on [protein|A25C1530DA7BB007A288E525404E9F775E219FE8|SpoIVA] [Pubmed|22171814]

Expression and Regulation

Operons

• genes: [gene|E9ADA4957F3DCF471851B742309258569007BC6C|yppG]
  description: [Pubmed|15383836]

  sigma factors

  • [protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK]: sigma factor, [Pubmed|26577401,15383836], in [regulon|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK regulon]
  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]: sigma factor, in [regulon|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE regulon]

  regulatory mechanism

  • [protein|19228BAD44E6DA3DE908DC390FDF628C18D94E65|GerE]: activation, [Pubmed|26577401], in [regulon|19228BAD44E6DA3DE908DC390FDF628C18D94E65|GerE regulon]

  regulation

  • expressed late during sporulation in the mother cell ([SW|SigE], [protein|search|SigK], [protein|search|GerE]) [Pubmed|26577401,15383836]

Biological materials

Mutant

• MGNA-A479 (yppG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/479 NBRP B. subtilis, Japan]
• BKE22250 (Δ[gene|E9ADA4957F3DCF471851B742309258569007BC6C|yppG]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE22250 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGCGATTCCCCCCTAAA, downstream forward: _UP4_TGATAGAAGCATGAATGAAT
• BKK22250 (Δ[gene|E9ADA4957F3DCF471851B742309258569007BC6C|yppG]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK22250 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGCGATTCCCCCCTAAA, downstream forward: _UP4_TGATAGAAGCATGAATGAAT

References

Reviews

• 27227299

Original publications

• 22171814,15383836,26577401